Identification and detection of genetically modified papaya resistant to papaya ringspot virus strains in Thailand.
نویسندگان
چکیده
Many lines of genetically modified (GM) papaya (Carica papaya Linnaeus) have been developed worldwide to resist infection from various strains of papaya ringspot virus (PRSV). We found an unidentified and unauthorized GM papaya in imported processed papaya food. Transgenic vector construct that provides resistance to the PRSV strains isolated in Thailand was detected. An original and specific real-time polymerase chain reaction method was generated to qualitatively detect the PRSV-Thailand-resistant GM papaya.
منابع مشابه
Complete Genome Sequence of Papaya Ringspot Virus Isolated from Genetically Modified Papaya in Hainan Island, China
The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. The virus shares 92% nucleotide sequence identity with the isolate that is unable to infect PRSV-resistant transgenic papaya.
متن کاملComplete genome sequence of an isolate of papaya leaf distortion mosaic virus from commercialized PRSV-resistant transgenic papaya in China.
Papaya leaf distortion mosaic virus is highly destructive to commercial papaya production. Here, the complete genome sequence was determined for an isolate of papaya leaf distortion mosaic virus, designated PLDMV-DF, infecting the commercialized papaya ringspot virus (PRSV)-resistant transgenic papaya from China. Excluding the 3'-poly (A) tail, the sequence shares high sequence identity to seve...
متن کاملPersistence and Bioavailability of Transgenic Genes Released from Genetically Modified Papaya and the Influence on Soil Bacterial Communities in Taiwan
Papaya ringspot virus (PRSV) is the most destructive disease of papaya (Carica papaya L.) in Taiwan, and transgenic papaya resistant to PRSV and PLDMV (papaya leaf distortion mosaic virus) were developed in Taiwan. Horizontal gene transformation (HGT) of kanamycin resistant gene nptII from transgenic papaya, the persistence of transgenic genes in soil samples, and the influence of transgenic pa...
متن کامل20 Years of Transgenic Research in China for Resistance to Papaya ringspot virus
Papaya is a favorite fruit and important industrial raw material in China. It is traditionally planted in southern China. Due to its wide uses and mystical health-related effects, papaya has been listed in fruits of high priority. Papaya ringspot virus (PRSV) is the main limitation of papaya production. PRSV infection rate reaches 100% in field in the late season of planting, which considerably...
متن کاملForbidden fruit: transgenic papaya in Thailand.
Dressed in white, hooded ‘‘personal protection suits,’’ Greenpeace activists donned goggles, gloves, and respiratory masks—the kind of dress you expect to see in the clean zone of a nanotechnology laboratory, not in a field in bucolic northeast Thailand. Easily bridging a barbed wire fence with a stepladder, they began pulling transgenic papaya (Carica papaya) from the trees, throwing the fruit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Biological & pharmaceutical bulletin
دوره 37 1 شماره
صفحات -
تاریخ انتشار 2014